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human starr seq screening vector  (Addgene inc)


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    Structured Review

    Addgene inc human starr seq screening vector
    Human Starr Seq Screening Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/starr+seq+screening+vector/hSTARR-seq_SCP1+vector_blocking+4+(Plasmid+%2399319)/pm35594906-509-3-11
    Average 90 stars, based on 3 article reviews
    human starr seq screening vector - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene # 71509 [ ]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units.
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene #71509 [30]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, Giresi et al ., 2007 ) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al ., 2013 . .. To asses enhancer activity, E14 cells were transfected with this plasmid library using a NucleofectorTM 2b device using the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001).

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: The negative (nc1 and nc2, GR responsive elements) and positive (CMV enhancer) control regions as well as RARα motif variants were ordered as gBlocks (IDT) and are listed in Table S1. .. DNA fragments were subsequently cloned into the STARR-seq screening vector (pSTARR-seq_human, Addgene plasmid #71509) using the In-Fusion ® HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: To exit from pluripotency and induce differentiation, LIF was withdrawn and retinoic acid (RA, Sigma, R2625) was added to the medium to a final concentration of 1 μM. .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ). .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ).

    Plasmid Preparation:

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene # 71509 [ ]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units.
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene #71509 [30]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: The negative (nc1 and nc2, GR responsive elements) and positive (CMV enhancer) control regions as well as RARα motif variants were ordered as gBlocks (IDT) and are listed in Table S1. .. DNA fragments were subsequently cloned into the STARR-seq screening vector (pSTARR-seq_human, Addgene plasmid #71509) using the In-Fusion ® HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: To exit from pluripotency and induce differentiation, LIF was withdrawn and retinoic acid (RA, Sigma, R2625) was added to the medium to a final concentration of 1 μM. .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ). .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ).

    Cloning:

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene # 71509 [ ]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: CRUP: a comprehensive framework to predict condition-specific regulatory units.
    Article Snippet: The negative (GR responsive element) and positive (CMV enhancer) control regions were ordered as gBlocks (IDT). .. DNA fragments were subsequently cloned into the STARR-seq screening vector (Addgene #71509 [30]) using In-Fusion HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #71509 ) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: The negative (nc1 and nc2, GR responsive elements) and positive (CMV enhancer) control regions as well as RARα motif variants were ordered as gBlocks (IDT) and are listed in Table S1. .. DNA fragments were subsequently cloned into the STARR-seq screening vector (pSTARR-seq_human, Addgene plasmid #71509) using the In-Fusion ® HD Cloning Kit (Takara/Clonetech). ..

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #7150938) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..

    Isolation:

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, Giresi et al ., 2007 ) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al ., 2013 . .. To asses enhancer activity, E14 cells were transfected with this plasmid library using a NucleofectorTM 2b device using the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001).

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: To exit from pluripotency and induce differentiation, LIF was withdrawn and retinoic acid (RA, Sigma, R2625) was added to the medium to a final concentration of 1 μM. .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ). .. As input material for the reporter screen, accessible chromatin from E14 mESCs treated with RA (Sigma, #R2625) for 4 h was isolated by formaldehyde-assisted isolation of regulatory elements (FAIRE, ( )) and subsequently cloned into the STARR-seq screening vector (Addgene #71509) following the protocol described in Arnold et al. ( ).

    other:

    Article Title: Assessing genome-wide dynamic changes in enhancer activity during early mESC differentiation by FAIRE-STARR-seq
    Article Snippet: The negative (nc1 and nc2, GR responsive elements) and positive (CMV enhancer) control regions as well as RARα motif variants were ordered as gBlocks (IDT) and are listed in .

    Generated:

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #71509 ) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #7150938) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..

    Nested PCR:

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #71509 ) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..

    Article Title: Pluripotency reprogramming by competent and incompetent POU factors uncovers temporal dependency for Oct4 and Sox2.
    Article Snippet: .. Cloning: Reporter plasmids were generated by cloning either gBlocks (IDT) or fragments obtained by nested PCR from genomic ESC DNA into the STARR-seq screening vector (Addgene #7150938) using In-Fusion® HD Cloning Kit (Takara, Clontech). ..



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